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The immunocytochemical detection of Herpes simplex virus in cervical smears—a valuable technique for routine use
Author(s) -
Adams Richard L.,
Springall David R.,
Levene Max M.,
Bushell Timothy E. C.
Publication year - 1984
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711430403
Subject(s) - herpes simplex virus , immunocytochemistry , immunostaining , virus isolation , virus , medicine , isolation (microbiology) , immunoperoxidase , pathology , virology , papanicolaou stain , antibody , biology , immunohistochemistry , immunology , cervical cancer , monoclonal antibody , microbiology and biotechnology , cancer
Herpes simplex virus type 2 is demonstrated in routine cervical smears by immunocytochemistry. Optimum antibody dilution and suitable fixation were determined and immunostaining results were found to compare favourably with those obtained by screening Papanicolaou‐stained smears or by virus culture methods (virus isolation). Results obtained for immunostaining of smears from 158 patients agreed with those of virus isolation in 47 of 50 cases where virus isolation was performed. It is suggested that this immunocytochemical technique is valuable in detection of Herpes simplex virus in cervical smears, offering an inexpensive and rapid laboratory diagnosis with advantage to both patient and clinician.