Premium
The reactivity of Reed–Sternberg cells with monoclonal antisera at thin section and ultrastructural levels
Author(s) -
Stuart A. E.,
Volsen S. G.,
Zola H.
Publication year - 1983
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711410108
Subject(s) - monoclonal , monoclonal antibody , ultrastructure , antiserum , microbiology and biotechnology , electron microscope , chemistry , biology , pathology , antigen , medicine , immunology , physics , antibody , optics
In an attempt to improve the morphology of Reed–Sternberg cells after immunochemical procedures, the biotin–avidin technique has been used. Satisfactory morphology was obtained at the level of both light and electron microscopy. Reed–Sternberg cells failed to react with a monoclonal antimonocyte serum but gave positive results with anti‐1a and FMC7, a monoclonal anti‐B cell serum. Positive results were also obtained with FMC 1 and B 1 both of which are monoclonal B cell antisera.