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Tissue culture of guinea‐pig gall‐bladder epithelium
Author(s) -
Elhamady M. S.,
Hopwood D.,
Milne G.,
Ross P.,
Bouchier I. A. D.
Publication year - 1983
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711400305
Subject(s) - gall , guinea pig , epithelium , new guinea , biology , tissue culture , anatomy , pathology , medicine , botany , endocrinology , in vitro , biochemistry , history , ethnology
Tissue culture of gall‐bladder was attempted in the following media: Dulbecco, Eagle's minimum essential medium, NCTC 135, medium 199 and Ham's F12. Growth occurred in all of them for up to 2 weeks assessed by light microscopy. No enhancement of growth was induced by collagenase trypsin insulin or hydrocortisone. Scanning electron microscopy confirmed that new cells colonised the free surface of the explant. Transmission electron microscopy showed good preservation of the original tall epithelial cells for the period of study. The new migrating cells were flatter, but retained the morphological features of the columnar cells. Secretory granules were absent after 1 day in culture but increased amounts of glycogen and lipid began to appear in the epithelium.