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The behaviour of human ameloblastoma tissue in cell culture
Author(s) -
Prime S. S.,
Sim F. R. P.,
Toh B. H.
Publication year - 1983
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711390404
Subject(s) - cytoplasm , staining , fluorescein isothiocyanate , biology , fluorescein , receptor , actin , fluorescence microscope , cell , microbiology and biotechnology , electron microscope , morphology (biology) , cell type , ultrastructure , pathology , chemistry , biophysics , fluorescence , anatomy , biochemistry , medicine , physics , quantum mechanics , genetics , optics
Human ameloblastoma tissue was investigated using cell culture techniques, transmission electron microscopy and fluorescent microscopy. Cultured cellular morphology was dependent on the type of substratum, with polygonal cells predominant on collagen substrata in contrast to an elongated cellular morphology on glass substrata. The presence of tonofilaments and desmosomes confirmed the epithelial origin of these cells. The distribution of Con A surface receptors and cytoplasmic actin in the same cell was studied using a double fluorochrome technique. Incubation with fluorescein isothiocyanate‐labelled Con A at 37°C for increasing time periods resulted in the Con A receptors showing progressive changes in staining patterns from clusters, to caps to perinuclear globules. Sequential changes in cytoplasmic actin, labelled by a specific anti‐actin auto‐antibody traced with rhodamine‐labelled goat anti‐human globulin, corresponded to the Con A staining patterns.