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Collagen production by human mesothelial cells in vitro
Author(s) -
Harvey W.,
Amlot P. L.
Publication year - 1983
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711390309
Subject(s) - mesothelial cell , basement membrane , serous fluid , mesothelium , in vitro , pathology , chemistry , type i collagen , microbiology and biotechnology , fibrosis , type iv collagen , cell type , biology , cell , extracellular matrix , medicine , biochemistry , laminin
Serous effusions from a variety of malignant and non‐malignant conditions were found to contain cells which had the morphological, histochemical and ultrastructural characteristics of mesothelial cells. In culture they were the predominant cell type and synthesised large quantities of the interstitial collagens type I and III, with the proportion of type III frequently approaching or exceeding 50 per cent. There was no evidence that they synthesised basement membrane collagen chains of type IV or type V. Since mesothelial cells are assumed to secrete the mesothelial basement membrane, the synthesis of interstitial collagens by desquamated mesothelial cells appears to reflect a change in the phenotype of collagen synthesis. We suggest that this change may be an important factor in the fibrosis of the serosal wall which frequently accompanies diseases with chronic serious effusion.