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Demonstration of amyloid A (AA) protein and amyloid P component (AP) in deposits of systemic amyloidosis associated with renal adenocarcinoma
Author(s) -
Hind C. R. K.,
Tennent Glenys A.,
Evans D. J.,
Pepys M. B.
Publication year - 1983
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711390207
Subject(s) - amyloidosis , amyloid (mycology) , pathology , immunohistochemistry , immunoperoxidase , aa amyloidosis , adenocarcinoma , serum amyloid p component , serum amyloid a protein , staining , medicine , chemistry , serum amyloid a , cancer , immunology , disease , antibody , inflammation , c reactive protein , monoclonal antibody , familial mediterranean fever
The fibril protein in three cases of systemic amyloidosis associated with renal adenocarcinoma was identified as amyloid A protein (AA) using immunohistochemical staining techniques. Amyloid P component was also present in all deposits. In one case unfixed snap frozen tissues were studied directly whilst in the other two, which were investigated retrospectively, fixed paraffin sections were stained by immuno‐fluorescence and immunoperoxidase methods after treatment with trypsin. Enzymatic digestion was necessary to restore reactivity with anti‐AP but anti‐AA reacted well without any such treatment. These findings establish that renal carcinoma, the carcinoma most frequently associated with systemic amyloidosis, causes the same type of secondary or reactive systemic amyloid as chronic infections and inflammatory disorders.