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The fate of plasma protein which escapes from blood vessels of the choroid plexus of the rat–an electron microscope study
Author(s) -
Hurley J. V.,
McD. Anderson R.,
Sexton P. T.
Publication year - 1981
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711340107
Subject(s) - choroid plexus , electron microscope , vacuole , stroma , cytoplasm , pathology , tight junction , choroid , microbiology and biotechnology , ferritin , biology , plexus , anatomy , chemistry , cell junction , immunohistochemistry , cell , medicine , endocrinology , retina , biochemistry , physics , neuroscience , optics , central nervous system
Fenestrated blood vessels in the rat choroid plexus are permeable to dye‐labelled proteins, HRP and ferritin. Most leakage appears to be via fenestrae but some additional escape of marker appears to take place through transient and reversible openings in the junctions between endothelial cells. After they have escaped into the choroidal stroma markers are prevented from entering the CSF by tight junctions between the epithelial cells which cover the choroid plexus, but how they are removed from the extravascular space is not known. Electron microscope study of rats who have been given multiple intravenous injections of ferritin shows that extravascular ferritin is taken up both by connective tissue cells in the choroidai stroma and by choroidal epithelial cells. The findings suggest that the ingested protein is subsequently broken down within lysosomal vacuoles in the cytoplasm of these cells. Such intracellular digestion may be the major means of controlling the protein content of the extravascular spaces of the choroid plexus.