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Acute and persistent ultrastructural changes in rat liver after a single dose of 2‐acetylaminofluorene: Effect of co‐administration of cycloheximide
Author(s) -
Flaks Bojan,
Basley Wilfrid A.
Publication year - 1980
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711310102
Subject(s) - cycloheximide , 2 acetylaminofluorene , administration (probate law) , ultrastructure , chemistry , medicine , pathology , protein biosynthesis , biochemistry , political science , in vitro , microsome , law
Electron microscope studies have been made of rat hepatic cell morphology, 24 hr, 48 hr and 24 weeks after a single intragastric dose of 2-acetylaminofluorene (2-AFF), alone or together with a single s.c. dose of cycloheximide (CHM). CHM prevents the acute perinuclear glycogen pooling, peripheral displacement of cytoplasmic organelles and nuclear shrinkage induced by 2-AAF. However, the induction by 2-AAF of changes in the granular endoplasmic reticulum, mitochondria and bile canaliculi is enhanced by co-administration of CHM. These changes persist for at least 24 weeks and are similar to those induced during 2-AAF hepatocarcinogenesis and observed in hepatocellular tumours. In view of the similar results obtained in previous studies with the azocarcinogen, 3'-methyl-4-dimethylaminoazobenzene, the possibility is discussed that these persistent changes might be associated with the initiation phase of neoplastic induction.