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Oesophageal neoplasia in male wistar rats due to parenteral di(2‐hydroxypropyl)nitrosamine (DHPN): A combined histopathological, histochemical and electron microscopic study
Author(s) -
Levison D. A.,
Hopwood D.,
Morgan R. G. H.,
Coghill G.,
Milne G. A.,
Wormsley K. G.
Publication year - 1979
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711290106
Subject(s) - nitrosamine , pathology , carcinoma , population , mucin , carcinogen , saline , basal cell , medicine , staining , intraperitoneal injection , biology , genetics , environmental health
Intraperitoneal di(2-hydroxypropyl)-Nitrosamine (DHPN) caused a high incidence of oesophageal squamous carcinoma in male Wistar rats, particularly in rats killed 11 or more months after the start of injections. No control rats (injected intraperitoneally with saline) developed an oesophageal neoplasms. Histopathologically, the tumours were moderately well differentiated. Histochemical studies showed minor increases in mucin staining and mast cell population and a marked increase in bacteria in tumour-bearing oesophaguses. Electron microscopy showed the tumours to be similar to, but to differ in some respects from squamous carcinomas at other sites in humans. The possible implications of this work for human disease are twofold. It could provide a model for further study of aspects of oesophageal carcinoma and it serves to remind us that all potential oesophageal carcinogens need not act during swallowing.