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The identification of particulate matter in biological tissues and fluids
Author(s) -
Crocker P. R.,
Dieppe P. A.,
Tyler Gina,
Chapman S. K.,
Willoughby D. A.
Publication year - 1977
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711210106
Subject(s) - araldite , electron microscope , particulates , microscopy , materials science , optical microscope , biological fluids , scanning electron microscope , biological specimen , chemistry , mineralogy , analytical chemistry (journal) , optics , nanotechnology , chromatography , composite material , physics , adhesive , organic chemistry , layer (electronics)
Sections, 1-2 micron thick, of Araldite embedded tissue were prepared to provide a link between optical and electron microscopy for the identification of crystals. This technique permits examination of individual particles by means of polarising light microscopy, electron microscopy, X-ray energy spectroscopy and electron diffraction. It provides a reliable method for routine and research studies. It has led to the positive identification of individual crystals of calcium pyrophosphate dihydrate and hydroxyapatite in synovial fluids. The method can be applied to the identification of other particulate matter in a variety of biological specimens.