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CD10 + stromal cells form B‐lymphocyte maturation niches in the human bone marrow
Author(s) -
Torlakovic Emina,
Tenstad Ellen,
Funderud Steinar,
Rian Edith
Publication year - 2005
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1705
Subject(s) - stromal cell , lymphopoiesis , bone marrow , lymph node stromal cell , population , b cell , pathology , lymphocyte , cd20 , immunology , biology , immunohistochemistry , microbiology and biotechnology , haematopoiesis , chemistry , medicine , stem cell , antibody , environmental health
In adult mammals, early B‐lymphopoiesis takes place in the bone marrow in close association with stromal cells. Both the phenotype of the stromal cells and the molecules involved in this essential interaction are as yet inadequately described. In this study, all benign, differentiating B‐cells (Pax‐5 + lymphoid cells) are shown, by using two‐colour immunohistochemistry on biopsies from human bone marrow, to be in close contact with scant dendritic CD10 + stromal cells until they leave via the sinusoids. This CD10 + stromal cell population does not fully overlap with the VCAM‐1 + stromal cell population. Furthermore, using a set of B‐cell differentiation markers (TdT, Pax‐5, and CD20), B‐cell development is shown to be spatially oriented, with maturation progressing towards bone marrow sinusoids. In conclusion, CD10 + stromal cells form distinct B‐lymphocyte maturation niches in the human bone marrow. Copyright © 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.