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Abnormal PcG protein expression in Hodgkin's lymphoma. Relation with E2F6 and NFκB transcription factors
Author(s) -
SánchezBeato Margarita,
Sánchez Esther,
García Juan F,
PérezRosado Alberto,
Montoya María C,
Fraga Mario,
Jesús Artiga M,
Navarrete Mercedes,
Abraira Víctor,
Morente Manuel,
Esteller Manel,
Koseki Haruhiko,
Vidal Miguel,
Piris Miguel A
Publication year - 2004
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1661
Subject(s) - bmi1 , transcription factor , polycomb group proteins , biology , downregulation and upregulation , transcription (linguistics) , microbiology and biotechnology , repressor , haematopoiesis , cancer research , chemistry , gene , genetics , stem cell , linguistics , philosophy
The Polycomb group (PcG) of proteins comprises a family of repressors of homeobox genes that play key roles in body formation, haematopoiesis and cell cycle control. In this study, a large‐scale analysis of PcG protein expression (BMI1, MEL18, PH1, RNF2, RING1, and RYBP) was performed in 321 Hodgkin's lymphoma (HL) biopsies and in reactive lymphoid tissues using tissue microarrays. The relevance of PcG proteins in HL was also investigated by the simultaneous analysis of PcG and other proteins involved in the control of cell cycle, transcription machinery and lymphoid differentiation. The analysis revealed increased expression of a set of PcG proteins (particularly RYBP and BMI1) in tumour cells in comparison with reactive lymphoid tissue. One of the most striking findings was anomalous RYBP expression in 55% of classical HL cases associated with an unfavourable response to treatment and shorter survival. The data obtained in this study also show an association of PcG proteins with E2F6 and NFκB transcription factors. The statistical relationship between PcG and NFκB activation was further explored in HL‐derived cell lines treated with curcumin, an NFκB inhibitor, and TNFα. Up‐ or downregulation of MEL18 was paralleled by loss or gain of activated NFκB, which suggests that NFκB may regulate expression of this protein. Investigation of the relationship between E2F6 and RING1 by immunofluorescence and confocal analysis, in HL cell lines and paraffin sections, revealed co‐expression of both proteins in the same tumour cells. These results allow us to propose that the formation of transcription complexes with E2F6 may modify the functional status of PcG proteins in HSR cells. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.