Premium
The wt1 ‐heterozygous mouse; a model to study the development of glomerular sclerosis
Author(s) -
Menke Aswin L,
IJpenberg Annemieke,
Fleming Stewart,
Ross Allyson,
Medine Claire N,
Patek Charles E,
Spraggon Lee,
Hughes Jeremy,
Clarke Alan R,
Hastie Nicholas D
Publication year - 2003
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1390
Subject(s) - albuminuria , glomerulosclerosis , nephrin , glomerular basement membrane , creatinine , podocyte , medicine , endocrinology , proteinuria , renal function , kidney
Abstract In the present study, it is shown that mice heterozygous for wt1 develop glomerular sclerosis and the nature and time course of events leading to the glomerular scarring are determined. Wt1 ‐heterozygous ( wt1 het) mice and their wild‐type littermates were closely monitored from birth and plasma levels of urea, creatinine, and albumin were compared with histological data and clinical features. One of the first indications of nephropathy in the wt1 het mouse was the development of proteinuria, accompanied by progressive elevation of the plasma levels of urea and creatinine. Subsequently, the mice developed albuminuria, which correlated with thickening of the glomerular basement membrane and fusion of the podocyte foot processes. Glomerulosclerosis was a relatively late event, accompanied by severe albuminuria and loss of WT1, nephrin, CD2AP, and α‐actinin‐4. Copyright © 2003 John Wiley & Sons, Ltd.