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Immunohistochemical detection of CD79a expression in precursor T cell lymphoblastic lymphoma/leukaemias
Author(s) -
Hashimoto Mitsuyoshi,
Yamashita Yoriko,
Mori Naoyoshi
Publication year - 2002
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1126
Subject(s) - immunohistochemistry , pathology , biology , thymoma , microbiology and biotechnology , medicine
Twenty‐three cases of precursor T cell lymphoid malignancies were examined with respect to CD79a expression. Immunohistochemical staining was performed on frozen tissue sections using a broad panel of antibodies and Southern blot analysis was undertaken using DNA probes encoding immunoglobulin heavy chain (IgH) gene and T‐cell receptor (TCR) β, γ and δ genes. Twelve (52%) of the 23 cases examined demonstrated CD79a expression. IgH and TCRβ, γ and δ gene rearrangements were found in 5, 9, 12 and 20 cases, respectively. CD79a‐positive neoplastic cells exhibited a phenotype and genotype characteristic of an early stage of T cell differentiation. Immunohistochemical staining was also performed on human thymus and thymoma to investigate the normality of CD79a expression, to discover that low‐level expression of CD79a is common in thymocytes and thymoma‐associated lymphocytes. These results suggest that CD79a is expressed weakly and transiently in immature T‐lineage cells. Copyright © 2002 John Wiley & Sons, Ltd.