Premium
Methylation‐specific PCR for detection of neoplastic DNA in biopsy washings
Author(s) -
Goessl Carsten,
Müller Markus,
Heicappell Rüdiger,
Krause Hans,
Schostak Martin,
Straub Bernd,
Miller Kurt
Publication year - 2002
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1063
Subject(s) - gstp1 , intraepithelial neoplasia , dna methylation , neoplastic transformation , biopsy , adenocarcinoma , medicine , precancerous condition , pathology , prostate cancer , prostate , methylation , mesothelioma , pca3 , malignant transformation , cancer , biology , dna , carcinogenesis , gene , genotype , gene expression , biochemistry , genetics
Epigenetic DNA alterations such as promoter hypermethylation of glutathione S‐transferase P1 ( GSTP1 ) in prostatic adenocarcinoma frequently constitute tumour biomarkers. Neoplastic transformation was identified in washings of prostate biopsies by GSTP1 promoter hypermethylation, using methylation‐specific PCR (MSP). Twenty‐six patients undergoing sextant transrectal prostate biopsies for clinically suspected prostate cancer were enrolled. All ten patients diagnosed with adenocarcinoma (100%) and four of six patients with prostatic intraepithelial neoplasia (67%), but none of ten patients with benign hyperplasia (0%), exhibited GSTP1 promoter hypermethylation in at least one out of six biopsy washings. Since this approach is transferable to various cancer entities, a sensitive and specific DNA‐based analysis of biopsy material seems generally feasible without impeding routine histopathological examination. Copyright © 2002 John Wiley & Sons, Ltd.