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Novel amphiphilic fluorescent graft copolymers: synthesis, characterization, and potential as gene carriers
Author(s) -
Wu Zhaoqiang,
Meng Lingzhi
Publication year - 2007
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.979
Subject(s) - copolymer , gel permeation chromatography , biocompatibility , materials science , monomer , amphiphile , agarose gel electrophoresis , polymer chemistry , micelle , fluorescence spectroscopy , dynamic light scattering , polymer , aqueous solution , fluorescence , organic chemistry , chemistry , nanotechnology , nanoparticle , dna , biochemistry , physics , quantum mechanics , metallurgy , composite material
Amphiphilic fluorescent graft copolymer (PVP‐PyATAm) was successfully synthesized by the free radical copolymerizations of hydrophobic monomer N ‐acryloyl‐thioureylene‐4‐(1‐pyrene)‐butyryl amide (PyATAm) with hydrophilic precursor polymers of vinyl‐functionalized poly ( N ‐vinylpyrrolidone) (Acryloyl‐PVP) in DMF. FT‐IR, 1 H NMR, TEM, gel permeation chromatography‐multi‐angle laser light scattering, UV‐vis spectroscopy, viscometric measurement, and fluorescence spectroscopy were used to characterize this copolymer. The TEM observation showed that the copolymer PVP‐ PyATAm formed spherical micelles in an aqueous solution and the size of micelles was between 50 and 70 nm in diameter. The interaction of PVP‐PyATAm copolymer and plasmid DNA was examined by agarose gel electrophoresis and TEM. Results indicated that the copolymer–DNA complexes were self‐assembled and the size of complexes was between 90 and 120 nm in diameter. Cytotoxity studies using MTT colorimetric assays suggested good biocompatibility of PVP‐PyATAm in vitro . These results suggested the potential of this graft copolymer as gene delivery carrier. Copyright © 2007 John Wiley & Sons, Ltd.