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Immune stimulating activity of an atrophic rhinitis vaccine associated to pegylated chitosan microspheres in vitro
Author(s) -
Jiang HuLin,
Park InKyu,
Kang MiLan,
Yoo HanSang,
Choi YunJaie,
Akaike Toshihiro,
Cho ChongSu
Publication year - 2007
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.861
Subject(s) - bordetella bronchiseptica , chitosan , materials science , ethylene glycol , nasal administration , microbiology and biotechnology , chemistry , medicine , pharmacology , biochemistry , biology , organic chemistry , genetics , bacteria
It has previously been found that chitosan microspheres are easily aggregated due to their physical and storage instabilities. In this study, to overcome their instability, chitosan was covalently conjugated with poly(ethylene glycol). Pegylated chitosan microspheres were prepared through the ionic gelation process of pegylated chitosan with tripolyphosphate. Bordetella bronchiseptica dermonecrotoxin, major virulence factor of atrophic rhinitis causative agent, was loaded onto pegylated chitosan microspheres for nasal vaccination. Average particle sizes of Bordetella bronchiseptica dermonecrotoxin‐loaded pegylated chitosan microspheres were 5.47 µm. Microspheres obtained from pegylated chitosan microspheres were physically more stable than those from chitosan microspheres, and Bordetella bronchiseptica dermonecrotoxin‐loaded pegylated chitosan microspheres released more Bordetella bronchiseptica dermonecrotoxin than Bordetella bronchiseptica dermonecrotoxin‐loaded chitosan microspheres in vitro . Macrophage RAW264.7 cells stimulated with Bordetella bronchiseptica dermonecrotoxin‐loaded pegylated chitosan microspheres gradually secreted tumor necrosis factor α and nitric oxide, suggesting that pegylated chitosan microspheres are very promising vaccine delivery systems. Copyright © 2007 John Wiley & Sons, Ltd.