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pH‐sensitive liposome retaining Fe‐porphyrin as SOD mimic for novel anticancer drug delivery system
Author(s) -
Kawakami Hiroyoshi,
Hiraka Kazue,
Tamai Miho,
Horiuchi Aiko,
Ogata Akihiko,
Hatsugai Tomomi,
Yamaguchi Aritomo,
Oyaizu Kenichi,
Yuasa Makoto
Publication year - 2007
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.855
Subject(s) - liposome , porphyrin , cytotoxicity , drug delivery , biophysics , superoxide dismutase , cationic liposome , phosphatidylcholine , endosome , chemistry , drug carrier , biochemistry , in vitro , enzyme , biology , organic chemistry , membrane , transfection , intracellular , phospholipid , gene
In this article the novel design of an anticancer drug delivery system is reported based on a pH‐sensitive liposome retaining the Fe‐porphyrin as a superoxide dismutase(SOD) mimic. The liposomes contained cationic/anionic lipid combinations and were composed of Fe‐porphyrin, L ‐ α ‐phosphatidylcholine (DMPC), dimethylditetradecylammonium bromide (DTDAB), sodispdum oleate (OA Na ), and Tween‐80. The size of the liposome was approximately 30 nm. The EC 50 value (the effective concentration of compound required to produce a 50% lethal dose against cells) of the liposome was found to be significantly smaller than that of cisplatin as the control drug, suggesting that the liposome showed a high cytotoxicity toward the cancer cells. This is due to the fact that the pH‐sensitive liposome rapidly corresponds to the acidic environments of the endosomes and is unstable, and the Fe‐porphyrin is delivered into the cytosol. This result suggests that O 2 .−may be useful as a target molecule to induce the selective death of cancer cells and that a pH‐sensitive liposome retaining Fe‐porphyrin as an SOD mimic is a new class of anticancer agent. Copyright © 2006 John Wiley & Sons, Ltd.