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The preparation of nano‐scope chitosan‐oligomer copper complexes and their interaction with DNA
Author(s) -
Wang RongMin,
He NaiPu,
He YuFeng,
Xie YunTao,
Wang YunPu,
Tsuchida Eishun
Publication year - 2005
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.627
Subject(s) - materials science , oligomer , chitosan , copper , nano , dna , scope (computer science) , nanotechnology , chemical engineering , polymer science , polymer chemistry , composite material , metallurgy , biochemistry , chemistry , engineering , computer science , programming language
Water‐soluble low molecular weight chitosan of nanometer level and its copper complexes were prepared, and characterized by IR spectra, elemental analysis and gel permeation chromatography (GPC). The modes and mechanism of these copper complexes interaction with DNA were studied by a fluorescent probe method and electrophoresis analysis. It is suggested that there are electrostatic and intercalation modes of copper complexes interacting with DNA. At first, the cationic complex electrostaticly binds to the negatively charged phosphate backbone of DNA, and then a portion of the complex intercalates between the base pairs on the DNA duplex strand. Copyright © 2005 John Wiley & Sons, Ltd.