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Reusable pectin‐coated magnetic nanosorbent functionalized with an aptamer for highly selective Hg 2+ detection
Author(s) -
Deepuppha Nunthiya,
Khadsai Sudarat,
Rutnakornpituk Boonjira,
Kielar Filip,
Rutnakornpituk Metha
Publication year - 2021
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.5254
Subject(s) - detection limit , aptamer , selectivity , streptavidin , adsorption , nuclear chemistry , materials science , nanoparticle , combinatorial chemistry , chromatography , chemistry , biotin , nanotechnology , organic chemistry , biochemistry , genetics , biology , catalysis
A pectin‐coated magnetite nanoparticle functionalized with Hg 2+ ‐specific aptamer was developed to obtain a magnetic nanosorbent having high selectivity toward Hg 2+ and facile reusability. The aptamer labeled with biotin was immobilized on pectin‐coated MNP conjugated with streptavidin through the specific biotin–streptavidin interaction to obtain an aptamer‐functionalized nanosorbent. Pectin coated on the particle improved its stability and dispersibility in water, while thymine in the aptamer played an important role as a recognition element, which can bind to the Hg 2+ target via T–Hg 2+ –T complexes. The nanosorbent showed a linear concentration, ranging from 2.8 to 700 μg/L, with the limit of detection of 0.8 ± 0.2 μg/L and limit of quantitation of 2.8 ± 0.2 μg/L for the detection of Hg 2+ . It showed high selectivity with good tolerance toward Hg 2+ in the presence of other metal ions. The system can be repeatedly used for up to 10 cycles of Hg 2+ adsorption with only slight changes in its performance. In addition, satisfactory recoveries were also obtained for the detection of Hg 2+ in real water samples. This nanosorbent might be a promising tool for extended applications in biological and analytical fields.