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A novel silk/PES hybrid nanofibrous scaffold promotes the in vitro proliferation and differentiation of adipose‐derived mesenchymal stem cells into insulin producing cells
Author(s) -
Hosseini Motaharesadat,
DadashiNoshahr Karim,
Islami Maryam,
Saburi Ehsan,
Nikpoor Amin R.,
Mellati Amir,
MossahebiMohammadi Majid,
Soleimanifar Fatemeh,
Enderami Seyed E.
Publication year - 2020
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.4912
Subject(s) - scaffold , mesenchymal stem cell , microbiology and biotechnology , stem cell , adipose tissue , tissue engineering , cellular differentiation , materials science , in vitro , extracellular matrix , cell culture , matrix (chemical analysis) , in vivo , biomedical engineering , biology , biochemistry , medicine , genetics , gene , composite material
Using stem cells to replace the lost beta cells is a hopeful strategy in the treatment of diabetic patients. Furthermore, during stem cell culture and therapy, it is a need to use a substrate to act as a supportive matrix to mimic 3D in vivo microenvironment. Therefore, in this study, human adipose‐derived stem cells were used to differentiate into insulin‐producing cells (IPCs) on a silk/polyethersulfone (PES) scaffold. After exposing to the differentiation media, 2D and 3D (silk/PES) cultured cells were gradually aggregated and formed spherical shaped clusters. The viability of cells was comparable in both 3D and 2D culture. As the results of gene expression assay in both RNA and protein level showed, the differentiation efficiency was higher in 3D culture. Furthermore, ELISA revealed that the release of C‐peptide and insulin was higher in 3D than 2D culture. It seems that silk/PES nanofibrous hybrid scaffold could provide an appropriate matrix to mimic in vivo microenvironment and therefore increases the IPC differentiation potency of stem cells.