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Lyophilized hemoglobin‐albumin cluster with disaccharides: Long‐term storable powder of artificial O 2 ‐carrier
Author(s) -
Funaki Ryosuke,
Iwasaki Hitomi,
Kashima Tomonori,
Komatsu Teruyuki
Publication year - 2020
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.4846
Subject(s) - hemoglobin , trehalose , human serum albumin , cluster (spacecraft) , materials science , hydrogen bond , sucrose , chromatography , nuclear chemistry , chemistry , molecule , biochemistry , organic chemistry , computer science , programming language
Core‐shell protein cluster comprising a hemoglobin (Hb) in the center and human serum albumins (HSA) at the exterior, Hb‐HSA 3 cluster, is an artificial O 2 ‐carrier designed for use as a red blood cell (RBC) substitute. Lyophilization of the Hb‐HSA 3 cluster solution ([Hb unit] = 5 g/dL) with sucrose and trehalose yielded stable pink powder, which can be stored for 2 years at 4°C. Addition of pure water to the powder regenerated the homogeneous solution. The O 2 ‐binding properties and oxyHb rate have been unaltered during the storage period. Infrared spectroscopic studies revealed that the hydrogen bond between protein and sugar stabilizes three‐dimensional structure of the cluster.