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In situ biomimetic formation of nano‐hydroxyapatite crystals on chitosan microspheres
Author(s) -
Huang Di,
Xu Mengjie,
Niu Lulu,
Pérez Montserrat,
Du Jingjing,
Wei Yan,
Hu Yinchun,
Lian Xiaojie,
Chen Weiyi
Publication year - 2020
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.4745
Subject(s) - materials science , scanning electron microscope , biomaterial , chitosan , microsphere , composite number , chemical engineering , biocompatible material , in situ , adhesion , nano , nanotechnology , nuclear chemistry , biomedical engineering , composite material , chemistry , organic chemistry , medicine , engineering
Chitosan (CS) is a biocompatible, noncytotoxic biomaterial used before as base material for composites. On the other hand, nano‐hydroxyapatite (nHA) is one of the main components of human bones, highly used for biomedical applications. In this work, CS microspheres were produced under a W/O emulsion system. CS microspheres with calcium ions were then exposed to Na 3 PO 4 solution. In situ biomimetic nHA crystals were formed on CS microspheres to generate 15.14 ± 3.15‐μm composite microspheres. The microspheres were subsequently seeded with MG63 osteoblasts to observe their cell responses. All microspheres were characterized via scanning electron microscopy (SEM), phase‐contrast photomicroscopy, and X‐ray diffraction (XRD) analysis. The results showed flake‐like shape and islet‐like growth of nHA depositions presented on the surface of the CS microspheres. In vitro tests indicated that the CS/nHA microparticles were not only biocompatible but also enhanced cell adhesion and elongation due to the in situ biomimetic synthesis method.