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Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly( l ‐lactic acid)/multi‐walled carbon nanotube scaffolds and naringenin
Author(s) -
Ghorbani Sadegh,
Eyni Hossein,
Khosrowpour Zahra,
Salari Asl Leila,
Shabani Ronak,
Nazari Hojjatollah,
Mehdizadeh Mehdi,
Ebrahimi Warkiani Majid,
Amjadi FatemehSadat
Publication year - 2019
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.4733
Subject(s) - spermatogenesis , reactive oxygen species , materials science , fourier transform infrared spectroscopy , biophysics , electrospinning , microbiology and biotechnology , carbon nanotube , chemistry , chemical engineering , nanotechnology , biology , polymer , endocrinology , engineering , composite material
Spermatogenesis is a process in which animals generate spermatozoa from spermatogonial stem cells (SSCs). Successful in vitro differentiation of SSCs towards spermatids holds a significant promise for regeneration of impaired spermatogenesis. The present study aims to evaluate the efficiency of a 3D culture containing naringenin on proliferation and differentiation potentials of mouse SSCs. In this study, multi‐walled carbon nanotubes (MWCNTs) were incorporated into poly( l ‐lactic acid) (PLLA) fibers via electrospinning technique. The fibrous PLLA/MWCNTs were studied by Fourier‐transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), water contact angle measurements, electrical conductivity, and mechanical properties. Next, the SSCs were seeded into the PLLA/MWCNTs scaffolds and exhibited preferable survival and differentiation efficiency to subsequent cell lines. To shed more light on this matter, the immunocytochemistry, reverse‐transcription polymerase chain reaction (RT‐PCR), and qRT‐PCR results showed that the aforementioned cells on the 3D fabrics overexpressed the C‐kit and SYCP3 proteins. In addition, the reactive oxygen species ( ROS ) measurement data demonstrated that naringenin, an effective antioxidant, plays an important role in in vitro spermatogenesis. Taken together, the results of this study revealed the synergistic effects of 3D scaffolds and naringenin for efficient spermatogenesis in laboratories.