Premium
Preparation of a multifunctional bacterial fertilizer by living azospirillum simplex cells immobilized on poly(sodium acrylate)
Author(s) -
Wang YunPu,
Chen NaLi,
Wang RongMin,
Xiong YuBing,
Zha RuiTao
Publication year - 2004
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.449
Subject(s) - desorption , nuclear chemistry , sodium , chromatography , chemistry , microbial inoculant , acrylate , phosphate buffered saline , adsorption , copolymer , organic chemistry , biology , polymer , horticulture , inoculation
A living azospirillum simplex cell (LASSC) was immobilized on poly(sodium acrylate) (PSA). The method of preparation of the immobilized‐cell (LASSC‐PSA) was studied. LASSC was immobilized on PSA in an immobilizing medium of Na 2 HPO 4 · 12H 2 O‐NaH 2 PO 4 · 2H 2 O (pH 7.0) buffer, sterile water, a solution of NaCl (0.85% m/m), cultured medium (pH 7.0), respectively. The desorption capacity of LASSC on LASSC‐PSA in sterile water was measured and when the Na 2 HPO 4 · 12H 2 O‐NaH 2 PO 4 · 2H 2 O (pH 7.0) buffer served as the immobilizing medium, the desorption capacity of LASSC on LASSC‐PSA was the highest. The rate (m/m) of PSA and LASSC (wet weight) also affected considerably the desorption capacity of LASSC on LASSC‐PSA in sterile water, and the best rate was 1 : 0.5. The desorption capacity of LASSC on LASSC‐PSA was 50% of that at the beginning and that was still 10 9 cfu/g on storage for 5 months when the rate (m/m) of PSA and LASSC (wet weight) was 1 : 0.5, using Na 2 HPO 4 · 12H 2 O‐NaH 2 PO 4 · 2H 2 O (pH 7.0) buffer as immobilizing medium. Copyright © 2004 John Wiley & Sons, Ltd.