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Polymeric micelleplexes for improved photothermal endosomal escape and delivery of siRNA
Author(s) -
Chen Gang,
Ding Ling,
Wu Pengkai,
Zhou Yiwen,
Sun Minjie,
Wang Kaikai,
Oupický David
Publication year - 2018
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.4372
Subject(s) - endosome , photothermal therapy , gene silencing , materials science , biophysics , cationic polymerization , rna interference , cationic liposome , in vitro , microbiology and biotechnology , cancer cell , small interfering rna , cell penetrating peptide , quenching (fluorescence) , transfection , cell , nanotechnology , cancer , chemistry , biochemistry , biology , rna , gene , fluorescence , polymer chemistry , genetics , physics , quantum mechanics
Effective endosomal escape is required for practical application of nucleic acid therapeutics. In this study, we prepared siRNA micelleplexes with photothermally triggered endosomal escape and improved gene silencing activity in vitro. The micelleplexes were prepared from cholesterol‐modified and CXCR4‐inhibiting poly(amido amine)s (PAMD‐Ch). Near‐infrared dye IR780 was encapsulated in cationic PMAD‐Ch micelles, which then were used to form IR780 micelleplexes with siRNA. The micelleplexes displayed improved gene silencing efficiency upon laser irradiation, which was attributed to enhanced endosomal escape because of the photothermal effects of the encapsulated IR780. The IR780 micelleplexes retained the CXCR4 antagonism and inhibition of cancer cell invasion of the parent PAMD. Overall, this study validates codelivery of IR780 in siRNA micelleplexes as promising photothermally controlled siRNA delivery approach.