Premium
Novel extracellular polymeric substances produced by Cupriavidus necator IPT 027 grown on glucose and crude glycerol originated from biodiesel
Author(s) -
Ribeiro Paulo Leonardo Lima,
Campos Márcio Inomata,
Druzian Janice Izabel
Publication year - 2017
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.3957
Subject(s) - glycerol , cupriavidus necator , xylose , food science , monosaccharide , fermentation , rhizopus oryzae , chemistry , biochemistry , organic chemistry , polyhydroxyalkanoates , biology , bacteria , genetics
The aim of this study was to evaluate the production and properties of novel extracellular polymeric substances (EPS) from glucose (EPS 1) and crude glycerol (CG) from castor beans oil (EPS 2). Cupriavidus necator IPT 027 cultivated with glucose and CG (35 °C, 150 rpm, 72 hr, pH 7, shaker) produced approximately 1.60 ± 0.01 and 2.83 ± 0.02 g l −1 EPS, respectively, with high‐molecular weight (3.89 × 10 5 and 1.89 × 10 7 Da) and constituted of different functional groups such as uronic acid, monosaccharides (glucose, mannose, arabinose, and fucose) and primary amine group. The composition of the fermentable substrate influenced the melting temperature (221.11 and 230.18 °C), crystallinity (34.36% and 37.11%), degradation temperature (255.06 and 296.62 °C) and morphology. EPS showed pseudoplastic non‐Newtonian fluid behavior in the aqueous solutions, presenting potential applicability biotechnological and industrial mainly in food industry as emulsifiers and biosurfactants. This is the first study on the production and characterization of EPS obtained by C. necator IPT 027 in culture with glucose and CG. Copyright © 2016 John Wiley & Sons, Ltd.