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Redox reaction of PEO modified cytochrome c adsorbed on the electrode in ion conductive PEO matrix analyzed with non‐contact optical waveguide spectroscopy
Author(s) -
Fujita Kyoko,
Ohno Hiroyuki
Publication year - 2003
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.359
Subject(s) - materials science , cytochrome c , redox , electrode , adsorption , ethylene oxide , indium tin oxide , oligomer , absorption spectroscopy , photochemistry , polymer chemistry , polymer , chemistry , organic chemistry , composite material , biochemistry , physics , copolymer , quantum mechanics , mitochondrion , metallurgy
Optical waveguide spectroscopy was used under non‐contact conditions to analyze the visible absorption spectrum of poly(ethylene oxide) (M w = 150) modified cytochrome c (PEO 150 ‐cyt.c) adsorbed on an indium tin oxide (ITO) glass electrode. The redox reactions of the adsorbed PEO 150 ‐cyt.c were dynamically measured in situ in PEO oligomers (M w = 200). It was confirmed that PEO modification of cyt.c is effective in maintaining the redox activity of the cyt.c after adsorption on the ITO glass electrode. The PEO 150 ‐cyt.c adsorbed on the electrode re‐dissolved gradually in PEO oligomer. Against this, PEO 1000 ‐cyt.c having longer PEO chains (M w = 1000) was found to be adsorbed stably on the electrode. Copyright © 2003 John Wiley & Sons, Ltd.