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Preparation, characterization, and bioavailability of a phosphorylated human‐like collagen calcium complex
Author(s) -
Zhu Chenhui,
Chen Yanru,
Deng Jianjun,
Xue Wenjiao,
Ma Xiaoxuan,
Hui Junfeng,
Fan Daidi
Publication year - 2015
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.3557
Subject(s) - bioavailability , calcium , fourier transform infrared spectroscopy , nuclear chemistry , absorption (acoustics) , circular dichroism , materials science , fluorescence , atomic absorption spectroscopy , quenching (fluorescence) , phosphate , chemistry , biophysics , biochemistry , chemical engineering , pharmacology , metallurgy , biology , physics , composite material , quantum mechanics , engineering
The aim of this study was to determine whether a phosphorylated human‐like collagen calcium (PHLC–Ca) complex showed a higher bioavailability than other calcium supplements. To improve the calcium‐binding ability of human‐like collagen (HLC), modified HLC was prepared by introducing a reactive phosphate group using a dry‐heating method, followed by the formation of PHLC–Ca in MOPS buffer. PHLC–Ca was characterized using atomic absorption spectrophotometry, Fourier transform infrared (FTIR), fluorescence quenching, circular dichroism (CD), and ultraviolet–visible absorption (UV–vis) spectroscopy. The calcium uptake test showed that the bioavailability of PHLC–Ca was higher than that of CaCl 2 and G‐Ca. Data from a mouse model of osteoporosis suggested that PHLC–Ca is superior for treating osteoporosis than CaCl 2 or G‐Ca. These results indicate that PHLC–Ca is a desirable calcium supplement for use in daily life and clinical application. Copyright © 2015 John Wiley & Sons, Ltd.