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Characterizations of damages of DNA caused by plasma treatment and reactive species formed thereby
Author(s) -
Kwon YoungWan,
Jin JungIl
Publication year - 2015
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.3530
Subject(s) - spectroscopy , atmospheric pressure plasma , electron paramagnetic resonance spectroscopy , fluorescence spectroscopy , raman spectroscopy , plasma , materials science , reactive oxygen species , characterization (materials science) , dna damage , circular dichroism , aqueous solution , electron paramagnetic resonance , analytical chemistry (journal) , fluorescence , photochemistry , nanotechnology , dna , nuclear magnetic resonance , chemistry , environmental chemistry , optics , biochemistry , physics , quantum mechanics
Plasma sources at atmospheric pressures have been studied to develop plasma treatment for a variety of biomedical applications. But in such systems, reactive oxygen nitrogen species (RONS) induce severe damage in cells. Here, we briefly introduce atmospheric pressure plasma sources, review recent progress in characterization tools, and present results for RONS in the interface, aqueous system, and cellular components obtained via optical emission spectroscopy, fluorescence indicators, and electron paramagnetic resonance spectroscopy. Gel electrophoresis, circular dichroism spectroscopy, and Raman spectroscopy are used to investigate the damage caused by RONS that reaches the central cellular components and severely modifies DNA. Copyright © 2015 John Wiley & Sons, Ltd.