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Dendritic polyglycerol with secondary amine shell as an efficient gene delivery vector with reduced toxicity
Author(s) -
Zhu Yu,
Hazeldine Stuart,
Li Jing,
Oupický David
Publication year - 2014
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.3331
Subject(s) - polyethylenimine , transfection , gene delivery , polymer , amine gas treating , cytotoxicity , materials science , toxicity , polymer chemistry , shell (structure) , biophysics , chemistry , organic chemistry , gene , biology , biochemistry , in vitro , composite material
Dendritic polycation (PG‐BEN) using polyglycerol as a core and secondary amine shell consisting of N 1 , N 11 ‐bisethylnorspermine (BEN) was synthesized. Polymers containing primary amines in the shell (PG‐Nor and PG‐NH 2 ) were synthesized as controls to allow evaluation of the shell effect on physicochemical and transfection properties of the polymers. All studied polymers condensed DNA and formed polyplexes with sizes less than 110 nm. PG‐BEN and PG‐Nor had a similar transfection activity that was fully comparable with that of the control polyethylenimine. Amongst the studied polymers, PG‐BEN demonstrated the lowest cytotoxicity, suggesting that PG‐BEN is a promising gene delivery vector with favorable transfection/toxicity profile. Copyright © 2014 John Wiley & Sons, Ltd.