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Enhanced hydration stability of Bombyx mori silk fibroin/PEG 600 composite scaffolds for tissue engineering
Author(s) -
Selmin Francesca,
Gennari Chiara G. M.,
Minghetti Paola,
Marotta Laura A.,
Viviani Barbara,
Vagdama Pankaj,
Montanari Luisa,
Cilurzo Francesco
Publication year - 2014
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.3282
Subject(s) - fibroin , materials science , chemical engineering , bombyx mori , peg ratio , silk , composite number , protein adsorption , composite material , polymer chemistry , chemistry , polymer , biochemistry , finance , economics , gene , engineering
Silk fibroin scaffolds obtained via regeneration by Ajisawa's reagent (CaCl 2 /H 2 O/EtOH, mole ratio 1:8:2) and freeze‐drying followed by steam sterilization resulted unstable when incubated in physiological medium over 1‐month period since the oxygen diffusivity and mechanical resistance to compression significantly decreased. Blending of regenerated silk fibroin with PEG 600 lead to a novel scaffold with an improved water stability. Multi‐parameter characterization has revealed that scaffolds made by composite material were stable in physiological media over at least 1 month of incubation. Interestingly, the presence of PEG has a notable impact on wettability and protein adsorption. This increase in hydrophilicity could be beneficial for initial cell adhesion and survival as well as for the adsorptive loading of growth factors. Copyright © 2014 John Wiley & Sons, Ltd.