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Molecular mass distribution of polycations and dextrans by high‐performance size exclusion chromatography
Author(s) -
Kumar Neeraj,
Azzam Tony,
Domb Abraham J.
Publication year - 2002
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.245
Subject(s) - pullulan , size exclusion chromatography , chromatography , molecular mass , molar mass distribution , materials science , degradation (telecommunications) , gel permeation chromatography , calibration , detector , analytical chemistry (journal) , chemistry , polymer , organic chemistry , polysaccharide , optics , composite material , computer science , telecommunications , statistics , mathematics , enzyme , physics
A high‐performance size exclusion chromatographic (HPSEC) method for molecular mass distribution (MMD) was developed for polycations. Results obtained from HPSEC using UV and RI detectors were compared with the viscosity measurements. Pullulan standards were used for M w calibration. It is concluded that HPSEC with a UV detector offers a sensitive and simple method that has the required accuracy. Degradation studies following this method indicate that dextrans are stable at a temperature of 55 °C and at different pH solutions for up to 7 days. Copyright © 2003 John Wiley & Sons, Ltd.