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Polypyrrole/multiwalled carbon nanotubes‐based biosensor for cholesterol estimation
Author(s) -
Singh K.,
Solanki Pratima R.,
Basu Tinku,
Malhotra B. D.
Publication year - 2012
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.2020
Subject(s) - cholesterol oxidase , polypyrrole , materials science , nanocomposite , biosensor , electrode , fourier transform infrared spectroscopy , nuclear chemistry , electrochemistry , indium tin oxide , cyclic voltammetry , thermal stability , polymer chemistry , chemical engineering , nanotechnology , organic chemistry , chemistry , layer (electronics) , engineering , enzyme
The nanocomposite electrode comprising of polypyrrole (PPY) and carboxy functionalized multiwalled carbon nanotubes (MWCNT) has been electrochemically fabricated onto indium–tin–oxide (ITO) electrode using p ‐toluene sulfonic acid (PTS). Cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) have been immobilized onto this PPY– MWCNT/ITO nanocomposite electrode using N ‐ethyl‐ N ‐(3‐dimethylaminopropyl) carbodiimide and N ‐hydroxy succinimide chemistry for estimation of esterified cholesterol. The ChEt–ChOx/PPY–MWCNT/PTS/ITO bioelectrode has been characterized using Fourier transform infrared spectroscopy, electrochemical techniques, and scanning electron microscope. This ChEt–ChOx/PPY–MWCNT/PTS/ITO nanobioelectrode has a response time of about 9 s, linearity of 4 × 10 −4 to 6.5 × 10 −3 M/l of cholesterol oleate concentration, K m of 0.02 mM, and thermal stability of upto 45°C. This electrode exhibits improved biosensing characteristics compared with other total cholesterol electrodes reported in literature till date and can be used to estimate cholesterol in blood serum samples. Copyright © 2011 John Wiley & Sons, Ltd.