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Coagulation of antibody‐sensitized latexes in the presence of antigen
Author(s) -
Ouali L.,
Stoll S.,
Pefferkorn E.,
Elaissari A.,
Lanet V.,
Pichot C.,
Mandrand B.
Publication year - 1995
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.1995.220060716
Subject(s) - coagulation , kinetics , polystyrene , antigen , antibody , size exclusion chromatography , materials science , chemistry , chromatography , immunology , medicine , biochemistry , physics , quantum mechanics , psychiatry , composite material , enzyme , polymer
The paper describes a study of the kinetics and mechanism of the coagulation of two types of immunoassays using sensitized latexes. The positive response to the first test is based on the aggregation of the gamma globulin (IgG)‐coated polystyrene latexes in the presence of IgM rheumatoid antigen. The second test is relative to the heteroaggregation of two types of sensitized latexes induced by the presence of human chorionic gonadotropin (HCG). In the latter test, two identical polystyrene latexes bearing carboxylic acid surface groups were sensitized by covalent coupling of monoclonal antibodies specific for the αHCG determinant on one type of latex and for the βHCG determinant on the other type. Using the Coulter Counter method, the aggregate size distribution c(n) was determined as a function of the number n of elementary constituents, thus enabling calculation of the number N(t) and weight S(t) average sizes of the aggregates. The temporal variations of the average sizes were compared with typical situtions of reaction‐limited aggregation processes in order to characterize the mechanism of aggregation induced by antibody–antigen reactions.