Prepation of poly( N ‐isopropylacrylamide)‐grafted silica gel and its temperature‐dependent interaction with proteins

Poly( N ‐isopropylacrylamide) oligomer was immobilized onto a silica gel surface. The gel adsorbed a hydrophobic protein γ‐globulin (IgG) at 37°C, however, did not adsorb IgG at 24°C. The adsorbed IgG at 37°C was adsorbed by lowering the temperatue, No adsorption of a hydrophilic protein bovin serum albumin (BSA) onto this matrix was observed at 37°C nor 24°C.