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In vitro growth and activity of chondrocytes on three dimensional polycaprolactone/chitosan scaffolds
Author(s) -
Jiang Xiaobing,
Lu Xiaoling,
Xiao Bo,
Wan Ying,
Zhao Yongxiang
Publication year - 2012
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.1830
Subject(s) - polycaprolactone , materials science , chitosan , swelling , biomedical engineering , in vitro , adhesion , compressive strength , scaffold , cartilage , composite material , biophysics , chemical engineering , chemistry , polymer , anatomy , biochemistry , medicine , biology , engineering
Porous polycaprolactone/chitosan blend scaffolds with various compositional proportions were prepared using a particulate‐leaching method. The pore parameters of resultant scaffolds were found to be mainly modulated by porogen. The compressive mechanical properties and hydrophilicity of scaffolds were examined by measuring their compressive modulus and stress strength as well as swelling index. Selected chondrocytes isolated from articular cartilage of knee joints of rabbits were seeded on these scaffolds, and further in vitro cultured for various periods. The growth and activity of seeded cells were estimated by counting numbers of cells proliferated on scaffolds and measuring the amounts of proteoglycans and type II collagen synthesized by the seeded cells. It was found that some scaffolds composed of proper component ratios and having appropriate pore parameters exhibited promising characteristics for the adhesion and proliferation of seeded cells while maintaining the phenotype and activity of the cells. Copyright © 2010 John Wiley & Sons, Ltd.