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Covalent VEGF protein immobilization on resorbable polymeric surfaces
Author(s) -
Edlund U.,
Sauter T.,
Albertsson A.C.
Publication year - 2011
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.1811
Subject(s) - covalent bond , carbodiimide , polymer chemistry , attenuated total reflection , acrylic acid , materials science , grafting , fourier transform infrared spectroscopy , polymer , allylamine , amine gas treating , hydrochloride , carboxylic acid , nuclear chemistry , chemistry , organic chemistry , chemical engineering , copolymer , infrared spectroscopy , polyelectrolyte , engineering
Vascular endothelial growth factor (VEGF) type protein, a potent angiogenic effector molecule, was successfully covalently immobilized onto the surfaces of the resorbable polymers poly( L ‐lactic acid) (PLLA) and poly( ε ‐caprolactone) through a three‐step strategy. The surfaces were first covalently grafted with poly(acrylic acid) using non‐destructive and solvent‐free vapor‐phase grafting. A diamine spacer was coupled to the carboxylic acid pendant groups on the graft chains using 1‐ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide hydrochloride/ N ‐hydroxysuccinimide chemistry and VEGF was finally covalently attached to the amine linkers. The chemistry and topography of the modified substrates were quantitatively and qualitatively verified with X‐ray photoelectron spectroscopy, attenuated total reflectance–Fourier transform infrared spectrometry, UV–VIS, scanning electron microscopy, and enzyme‐linked immunosorbent assay. Copyright © 2010 John Wiley & Sons, Ltd.