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Multi‐responsive methylcellulose/Fe‐alginate‐g‐PVA/PVA/Fe 3 O 4 microgels for immobilizing enzyme
Author(s) -
Xiao Congming,
Ma Peipei,
Geng Nannan
Publication year - 2011
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.1701
Subject(s) - vinyl alcohol , chemical engineering , materials science , adsorption , ferrous , magnetite , immobilized enzyme , polymer chemistry , ionic bonding , ion , enzyme , chemistry , polymer , organic chemistry , composite material , metallurgy , engineering
To take advantage of the respective character of methylcellulose (MC), poly(vinyl alcohol) (PVA), and alginate, novel physically cross‐linked methylcellulose/Fe‐alginate‐g‐PVA/PVA (MC/Fe‐Alg‐g‐PVA/PVA) microgels were prepared by emulsification/thermal gelation/freezing–thawing/ionic cross‐linking technique. Subsequently, some ferrous ions bound in the microgels were transformed into magnetite via in situ self oxidation. A model enzyme α ‐amylase was immobilized into microgels by direct adsorption. The release behavior of α ‐amylase indicated that the obtained complex microgels were magnetic‐, temperature‐, and pH‐ triple sensitive. Copyright © 2010 John Wiley & Sons, Ltd.