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A novel preparation method for microspheres by glycerol modified solid‐in‐oil‐in‐water multi‐emulsion
Author(s) -
Yuan Weien,
Ren Tiantian,
Wu Fei,
Zhao Hao,
Jin Tuo
Publication year - 2010
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.1438
Subject(s) - glycerol , plga , emulsion , dextran , materials science , chemical engineering , glycolic acid , phase (matter) , microsphere , drug delivery , chromatography , lactic acid , chemistry , nanotechnology , organic chemistry , nanoparticle , biology , bacteria , engineering , genetics
Biodegradable material poly( D, L ‐lactic‐co‐glycolic) acid (PLGA) plays an important role in drug‐sustained release systems. Here, we describe a glycerol modified solid‐in‐oil‐in‐water (m‐S/O/W) emulsion method for PLGA microspheres, in order to encapsulate proteins in PLGA by utilizing dextran glassy particles to protect the proteins from denaturing, unfolding, and aggregation during preparation and new external water phase to prevent the inner dextran glassy particles from leaking into the external water phase. External water phase containing 20, 40, 60, 80% glycerol showed that proteins released faster and more completely with increased glycerol content. According to their varied release profiles, microspheres of different formulations could be used to encapsulate vaccines or for delivering proteins over long‐term. Copyright © 2009 John Wiley & Sons, Ltd.