Premium
Surface plasmon resonance study on binding interactions of multivalent cyclophane hosts with immobilized guests
Author(s) -
Hayashida Osamu,
Sato Daisuke
Publication year - 2010
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.1398
Subject(s) - cyclophane , surface plasmon resonance , pentamer , dissociation constant , ethylamine , chemistry , molecular binding , dissociation (chemistry) , molecule , stereochemistry , materials science , organic chemistry , nanotechnology , nanoparticle , biochemistry , receptor
Guest‐binding affinities of water‐soluble cyclophane heptadecamer (1) and pentamer (2) with immobilized guests such as 1‐pyrenylmethylamine (PMA) and 2‐(1‐ naphthyl)ethylamine (NEA) were investigated by surface plasmon resonance (SPR) measurements. As a typical example, the binding constants ( K ) for 1 and 2 with the immobilized PMA as a guest were evaluated to be 2.5 × 10 7 and 2.7 × 10 6 M −1 , respectively, and were much larger than that of a monocyclic reference cyclophane ( K , 2.5 × 10 4 M −1 ). Interestingly, in the complexation of 1 and 2 with the immobilized guests, more favorable association and dissociation rate constant values ( k a and k d , respectively) were observed in comparison with those for the monocyclic cyclophane, reflecting multivalent effects in macrocycles. The multivalent effects in macrocycles as well as molecular recognition abilities of the cyclophane oligomers were confirmed even when the guest molecules were immobilized on SPR sensor chip surfaces. Copyright © 2009 John Wiley & Sons, Ltd.