Premium
Removal of lipopolysaccharide and reactive oxygen species using sialic acid immobilized polysulfone dialyzer
Author(s) -
Chang JungJhih,
Lin PoJu,
Yang MingChien,
Chien ChiangTing
Publication year - 2009
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.1309
Subject(s) - polysulfone , membrane , thiobarbituric acid , sialic acid , oxidative stress , hollow fiber membrane , fiber , adhesion , materials science , lipopolysaccharide , reactive oxygen species , covalent bond , acrylic acid , chemistry , polymer chemistry , biochemistry , nuclear chemistry , chromatography , polymer , organic chemistry , lipid peroxidation , monomer , biology , endocrinology
Abstract Sialic acid ( N ‐acetylneuraminic acid, NANA) was covalently immobilized onto the surface of a polysulfone (PSF) hollow fiber membrane. Prior to the immobilization, the surface of PSF was treated with ozone, followed by grafting with acrylic acid, and then the esterification of NANA. The surface concentration of NANA was determined by 2‐thiobarbituric acid (TBA) test. Hemocompatibility, the capability of suppressing oxidative stress, and clearance of lipopolysaccharide (LPS) from the resulting hollow fiber membrane were evaluated. The results show that by immobilizing NANA onto PSF hollow fiber, the adhesion of platelet was reduced, while both APTT and PT were little affected. Furthermore, oxidative stress was suppressed by NANA‐immobilized PSF hollow fibers. The level of LPS was also greatly reduced. Copyright © 2009 John Wiley & Sons, Ltd.