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Selective precipitation of plasmid with a water‐soluble cationic surfactant
Author(s) -
Ataci Nese,
Correia Isabell,
Arisan Inci,
Marcos João Carlos
Publication year - 2009
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.1245
Subject(s) - cationic polymerization , pulmonary surfactant , peg ratio , tallow , polyethylene glycol , nuclear chemistry , fourier transform infrared spectroscopy , glycerol , chemistry , chromatography , materials science , organic chemistry , chemical engineering , biochemistry , finance , engineering , economics
The aim of the present study was the selective precipitation of plasmid directly from alkaline lysate produced from bacterial culture with the cationic detergent, N , N ', N '‐polyethylene (10)‐ N tallow‐1, 3‐di amine propane. Polyethylene groups of peg‐10 tallow were functionalized with tertiary amine groups and long alkyl chain. The functional groups of cationic detergent were determined by means of 1 H nuclear magnetic resonance (NMR) spectroscopy. A simple and efficient process for plasmid purification by cationic detergent was found to be dependent on acid concentration. The structure of peg‐10 tallow cationic detergent after adding acid solution was characterized using Fourier transform infrared (FT‐IR) spectroscopy. Inorganic acid such as HCl was introduced into the solution of peg‐10 tallow. Controlling pH of precipitation system by the addition of different concentrations of HCl acid solutions on peg‐10 tallow improved the partial separation of RNA from plasmid DNA (pDNA). However, different lysate masses were applied to observe the effect of 7.5% acid solution on the separation of RNA from pDNA with a peg‐10 tallow cationic surfactant. The results were realized by 1% agarose gel analysis. Copyright © 2009 John Wiley & Sons, Ltd.