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Fabrication and properties of mineralized collagen‐chitosan/hydroxyapatite scaffolds
Author(s) -
Zhao Haiguang,
Ma Lie,
Gao Changyou,
Shen Jiacong
Publication year - 2008
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.1174
Subject(s) - chitosan , biopolymer , materials science , mineralization (soil science) , simulated body fluid , diffractometer , chemical engineering , biomineralization , composite number , scanning electron microscope , microstructure , mineralized tissues , thermogravimetric analysis , phosphate , scaffold , porosity , hydroxyapatites , composite material , calcium , chemistry , polymer , biomedical engineering , dentin , organic chemistry , metallurgy , medicine , nitrogen , engineering
A hydroxyapatite (HAp)/biopolymer composite scaffold was fabricated by mineralizing a crosslinked collagen/chitosan, which was pre‐mineralized with Ca 2+ and phosphate salts, in simulated body fluid (SBF) for only 24 hr. A self‐organized structure similar to bone is expected. Microstructures of the crosslinked collagen/chitosan scaffold, the pre‐mineralized collagen–chitosan scaffold (CCS), and the mineralized collagen‐chitosan/HAp scaffolds (MCCHS) were characterized by scanning electron microscopy (SEM), revealing non‐alteration of the porous structure and formation of the HAp particles. X‐ray diffractometer (XRD) confirmed the crystalline structure of the HAp. Thermal gravimetric analysis found that more HAp particles were formed when the CCSs were pre‐mineralized in a higher concentration of Ca 2+ . Water‐uptake ratio of the crosslinked CCS was ∼160, decreased to ∼120 after incubating in Ca 2+ solution, and further decreased to ∼20 after mineralization. Mechanical strength of the CCS was improved significantly after the in situ mineralization too. The method introduced here may be potentially applied to obtain other biopolymer/HAp composite in a short period. Copyright © 2008 John Wiley & Sons, Ltd.