Premium
In vitro biocompatibility of three‐dimensional chitosan scaffolds immobilized with chondroitin‐6‐sulfate
Author(s) -
Jou ChiHsiung,
Chen WeiChun,
Yang MingChien,
Hwang MouChen,
Chou WenLi,
Lin ShangMing,
Hsu ChengYi
Publication year - 2008
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/pat.1020
Subject(s) - chitosan , chondroitin sulfate , glutaraldehyde , biocompatibility , materials science , scanning electron microscope , chemical engineering , nuclear chemistry , ultimate tensile strength , biomedical engineering , composite material , chromatography , chemistry , biochemistry , glycosaminoglycan , medicine , engineering , metallurgy
Cellular‐compatible scaffolds were prepared using a three‐dimensional micro‐porous chitosan (CS) non‐woven fabric immobilized by glutaraldehyde (GA), followed by the immobilization of chondroitin‐6‐sulfate (ChS). To characterize the immobilizing process, tensile analysis, and scanning electron microscopy (SEM) were performed. The cell seeding efficiency and proliferation test were evaluated using L929 fibroblasts. The chitosan scaffolds showed high water vapor transmission rate and antibacterial activity. In addition, ChS‐immobilized scaffolds exhibited higher cell seeding efficiency and fibroblasts proliferation. These results demonstrated that the CS non‐woven fabrics grafted with GA and immobilized with ChS could be an appropriate candidate for wound healing and artificial scaffolds in the clinical applications. Copyright © 2007 John Wiley & Sons, Ltd.