Open AccessTowards a Fully Integrated Lab‐On‐A‐Chip
Author(s) -
Nestler Joerg,
Peter Harald,
Bier Frank
Publication year - 2018
Publication title -
optik & photonik
Language(s) - English
Resource type - Journals
eISSN - 2191-1975
pISSN - 1863-1460
DOI - 10.1002/opph.201800004
Subject(s) - cartridge , chip , dna microarray , lab on a chip , identification (biology) , point of care , microarray , sample (material) , barcode , microfluidics , sample preparation , computer hardware , fluorescence , nanotechnology , computer science , chromatography , materials science , chemistry , biology , physics , optics , telecommunications , medicine , gene , biochemistry , gene expression , botany , nursing , metallurgy , operating system
Abstract The identification of pathogens in body fluids is important for proper treatment of infections with antibiotics. Besides the pure identification of the pathogen's species, however, its antibiotic profile can be crucial with regard to their resistance to certain drugs. By the use of DNA‐based microarrays, multiple of such parameters can be measured at the same time, e.g., by fluorescence imaging. Before the optical detection, however, sample preparation and labeling steps have to be performed which require the controlled transport of liquid reagents and the sample. To perform these steps at the point of care by a portable instrument, a fully‐integrated cartridge technology is presented which can perform all the required pumping and heating steps in a chip‐card‐like format, followed by fluorescence readout of the microarray.