Open AccessImaging of Living Embryos
Author(s) -
Briggs Stephan
Publication year - 2013
Publication title -
optik & photonik
Language(s) - English
Resource type - Journals
eISSN - 2191-1975
pISSN - 1863-1460
DOI - 10.1002/opph.201300034
Subject(s) - confocal , confocal microscopy , microscopy , wavelength , optics , microscope , excitation wavelength , excitation , living systems , resolution (logic) , materials science , biomedical engineering , optoelectronics , physics , computer science , medicine , artificial intelligence , quantum mechanics
Multiphoton Microscopy is an advanced imaging technique for biological material that differs from standard confocal approaches. Multiphoton systems are the preferred choice for living, cellular imaging as it utilizes longer infrared wavelengths for excitation as opposed to shorter UV blue wavelengths that are more harmful to cellular tissue. This is done by taking advantage of the vibrational energy states of living tissue and results in an emission signal that is shorter than the excitation wavelengths. Multiphoton microscopy does not have the level of resolution that confocal microscopy has, but users are able to image deeper into living specimens which is extremely beneficial for non‐invasive biopsies performed in vivo . One application that truly demonstrates the benefits and differences between the two techniques is the imaging and analysis of living embryos.