Open AccessUltrasound‐Triggered Delivery of Iproplatin from Microbubble‐Conjugated Liposomes
Author(s) -
Browning Richard,
Thomas Nia,
Marsh Laura K.,
Tear Louise R.,
Owen Joshua,
Stride Eleanor,
Farrer Nicola J.
Publication year - 2021
Publication title -
chemistryopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 29
ISSN - 2191-1363
DOI - 10.1002/open.202100222
Subject(s) - liposome , microbubbles , carboplatin , drug delivery , ultrasound , prodrug , drug carrier , chemistry , biomedical engineering , biophysics , materials science , nanotechnology , medicine , chemotherapy , cisplatin , surgery , biochemistry , biology , radiology
The Pt IV prodrug iproplatin has been actively loaded into liposomes using a calcium acetate gradient, achieving a 3‐fold enhancement in drug concentration compared to passive loading strategies. A strain‐promoted cycloaddition reaction (azide‐ dibenzocyclooctyne) was used to attach iproplatin‐loaded liposomes L(Pt) to gas‐filled microbubbles (M), forming an ultrasound‐responsive drug delivery vehicle [M−L(Pt)]. Ultrasound‐triggered release of iproplatin from the microbubble‐liposome construct was evaluated in cellulo. Breast cancer (MCF‐7) cells treated with both free iproplatin and iproplatin‐loaded liposome−microbubbles [M−L(Pt)] demonstrated an increase in platinum concentration when exposed to ultrasound. No appreciable platinum uptake was observed in MCF‐7 cells following treatment with L(Pt) only or L(Pt)+ultrasound, suggesting that microbubble‐mediated ultrasonic release of platinum‐based drugs from liposomal carriers enables greater control over drug delivery.