Open AccessSolution‐Based Determination of Dissociation Constants for the Binding of Aβ42 to Antibodies
Author(s) -
Zhang Tao,
NagelSteger Luitgard,
Willbold Dieter
Publication year - 2019
Publication title -
chemistryopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 29
ISSN - 2191-1363
DOI - 10.1002/open.201900167
Subject(s) - microscale thermophoresis , chemistry , dissociation constant , monoclonal antibody , monomer , dissociation (chemistry) , antibody , affinities , analyte , microscale chemistry , ultracentrifuge , biophysics , chromatography , polymer , stereochemistry , biochemistry , organic chemistry , immunology , biology , receptor , mathematics education , mathematics
Amyloid β‐peptides (Aβ) play a major role in the pathogenesis of Alzheimer's disease. Therefore, numerous monoclonal antibodies against Aβ have been developed for basic and clinical research. The present study applied fluorescence based analytical ultracentrifugation and microscale thermophoresis to characterize the interaction between Aβ42 monomers and three popular, commercially available antibodies, namely 6E10, 4G8 and 12F4. Both methods allowed us to analyze the interactions at low nanomolar concentrations of analytes close to their dissociation constants ( K D ) as required for the study of high affinity interactions. Furthermore, the low concentrations minimized the unwanted self‐aggregation of Aβ. Our study demonstrates that all three antibodies bind to Aβ42 monomers with comparable affinities in the low nanomolar range. K D values for Aβ42 binding to 6E10 and 4G8 are in good agreement with formerly reported values from SPR studies, while the K D for 12F4 binding to Aβ42 monomer is reported for the first time.