Open AccessSelf‐Assembly of G‐Rich Oligonucleotides Incorporating a 3′–3′ Inversion of Polarity Site: A New Route Towards G‐Wire DNA Nanostructures
Author(s) -
Oliviero Giorgia,
D'Errico Stefano,
Pinto Brunella,
Nici Fabrizia,
Dardano Principia,
Rea Ilaria,
De Stefano Luca,
Mayol Luciano,
Piccialli Gennaro,
Borbone Nicola
Publication year - 2017
Publication title -
chemistryopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 29
ISSN - 2191-1363
DOI - 10.1002/open.201700024
Subject(s) - dna origami , nanostructure , oligonucleotide , nanotechnology , stacking , dna nanotechnology , dna , g quadruplex , homogeneous , materials science , inversion (geology) , chemistry , combinatorial chemistry , biology , mathematics , biochemistry , combinatorics , paleontology , organic chemistry , structural basin
Obtaining DNA nanostructures with potential applications in drug discovery, diagnostics, and electronics in a simple and affordable way represents one of the hottest topics in nanotechnological and medical sciences. Herein, we report a novel strategy to obtain structurally homogeneous DNA G‐wire nanostructures of known length, starting from the short unmodified G‐rich oligonucleotide d(5′‐CGGT‐3′–3′‐GGC‐5′) ( 1 ) incorporating a 3’–3′ inversion of polarity site. The reported approach allowed us to obtain long G‐wire assemblies through 5′–5′ π–π stacking interactions in between the tetramolecular G‐quadruplex building blocks that form when 1 is annealed in the presence of potassium ions. Our results expand the repertoire of synthetic methodologies to obtain new tailored DNA G‐wire nanostructures.